dtx k Search Results


dtx k  (Alomone Labs)
94
Alomone Labs dtx k
Aa, Kv1.1 antibody (1:25 dilution) labelled cell somata and their processes; the large, asymmetrical nuclei of these cells were unlabelled. Ab, the same field of view as panel Aa labelled with NF200 (1:100). NF200 antibody was used as a marker to identify neurons. Kv1.1 exclusively labelled the cells identified as neurons, but not all of the NF200-stained neurons were co-labelled with Kv1.1. Ac, double exposure of the Kv1.1 and NF200 fluorescent images shown above. The arrow in each panel (a-c) shows an example of a cell identified with NF200 that did not co-label with Kv1.1. The calibration bar in the lower right of the figure equals 20 μm and applies to all of the panels. B, example traces from a voltage-clamp recording made from an acutely isolated spiral ganglion neuron under control conditions and after exposure to 100 <t>nm</t> <t>DTX-K.</t> The scale bar applies to both sweeps. C, APmax was significantly greater following exposure to DTX-K. Asterisks indicate a statistically significant difference between control and DTX-K treated neurons (n = 5, P < 0.01). D, an average activation curve was calculated from measured current and injected voltage levels from five acute experiments assuming a reversal potential of −80 mV. Data were normalized to the maximum conductance of the low voltage activated component. Boltzmann fits were made as described in the legend for Fig. 6. For the single fit, V1/2 (half-maximal voltage) was −59 mV and k (slope) was 5.7. The 95 % confidence intervals for these two parameters were −61 to −57 mV and 3.9 to 7.5, respectively. For the double fit, the V1/2 (half-maximal voltage) values were −59.5 and 0.1 mV, for the low and high voltage fits, respectively (95 % confidence intervals: −61.7 to −57.3 mV and −3.0 to 3.2). The k values were 5.3 and 7.6 for the low and high voltage fits, respectively (95 % confidence intervals: 3.5 to 7.1 and 4.6 to 10.6).
Dtx K, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dtx+k/Dendrotoxin-K/pmc02290456-65-2-6
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dtx k - by Bioz Stars, 2026-10
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dtx-k  (CH Instruments)
90
CH Instruments dtx-k
Aa, Kv1.1 antibody (1:25 dilution) labelled cell somata and their processes; the large, asymmetrical nuclei of these cells were unlabelled. Ab, the same field of view as panel Aa labelled with NF200 (1:100). NF200 antibody was used as a marker to identify neurons. Kv1.1 exclusively labelled the cells identified as neurons, but not all of the NF200-stained neurons were co-labelled with Kv1.1. Ac, double exposure of the Kv1.1 and NF200 fluorescent images shown above. The arrow in each panel (a-c) shows an example of a cell identified with NF200 that did not co-label with Kv1.1. The calibration bar in the lower right of the figure equals 20 μm and applies to all of the panels. B, example traces from a voltage-clamp recording made from an acutely isolated spiral ganglion neuron under control conditions and after exposure to 100 <t>nm</t> <t>DTX-K.</t> The scale bar applies to both sweeps. C, APmax was significantly greater following exposure to DTX-K. Asterisks indicate a statistically significant difference between control and DTX-K treated neurons (n = 5, P < 0.01). D, an average activation curve was calculated from measured current and injected voltage levels from five acute experiments assuming a reversal potential of −80 mV. Data were normalized to the maximum conductance of the low voltage activated component. Boltzmann fits were made as described in the legend for Fig. 6. For the single fit, V1/2 (half-maximal voltage) was −59 mV and k (slope) was 5.7. The 95 % confidence intervals for these two parameters were −61 to −57 mV and 3.9 to 7.5, respectively. For the double fit, the V1/2 (half-maximal voltage) values were −59.5 and 0.1 mV, for the low and high voltage fits, respectively (95 % confidence intervals: −61.7 to −57.3 mV and −3.0 to 3.2). The k values were 5.3 and 7.6 for the low and high voltage fits, respectively (95 % confidence intervals: 3.5 to 7.1 and 4.6 to 10.6).
Dtx K, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dtx+k/dtx+k/pm26609114-167-5-21
Average 90 stars, based on 1 article reviews
dtx-k - by Bioz Stars, 2026-10
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pcdna3.1-dtxk-lumitoxin (plasmid #51691)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Aa, Kv1.1 antibody (1:25 dilution) labelled cell somata and their processes; the large, asymmetrical nuclei of these cells were unlabelled. Ab, the same field of view as panel Aa labelled with NF200 (1:100). NF200 antibody was used as a marker to identify neurons. Kv1.1 exclusively labelled the cells identified as neurons, but not all of the NF200-stained neurons were co-labelled with Kv1.1. Ac, double exposure of the Kv1.1 and NF200 fluorescent images shown above. The arrow in each panel (a-c) shows an example of a cell identified with NF200 that did not co-label with Kv1.1. The calibration bar in the lower right of the figure equals 20 μm and applies to all of the panels. B, example traces from a voltage-clamp recording made from an acutely isolated spiral ganglion neuron under control conditions and after exposure to 100 nm DTX-K. The scale bar applies to both sweeps. C, APmax was significantly greater following exposure to DTX-K. Asterisks indicate a statistically significant difference between control and DTX-K treated neurons (n = 5, P < 0.01). D, an average activation curve was calculated from measured current and injected voltage levels from five acute experiments assuming a reversal potential of −80 mV. Data were normalized to the maximum conductance of the low voltage activated component. Boltzmann fits were made as described in the legend for Fig. 6. For the single fit, V1/2 (half-maximal voltage) was −59 mV and k (slope) was 5.7. The 95 % confidence intervals for these two parameters were −61 to −57 mV and 3.9 to 7.5, respectively. For the double fit, the V1/2 (half-maximal voltage) values were −59.5 and 0.1 mV, for the low and high voltage fits, respectively (95 % confidence intervals: −61.7 to −57.3 mV and −3.0 to 3.2). The k values were 5.3 and 7.6 for the low and high voltage fits, respectively (95 % confidence intervals: 3.5 to 7.1 and 4.6 to 10.6).

Journal:

Article Title: Dendrotoxin-sensitive K + currents contribute to accommodation in murine spiral ganglion neurons

doi: 10.1113/jphysiol.2002.017202

Figure Lengend Snippet: Aa, Kv1.1 antibody (1:25 dilution) labelled cell somata and their processes; the large, asymmetrical nuclei of these cells were unlabelled. Ab, the same field of view as panel Aa labelled with NF200 (1:100). NF200 antibody was used as a marker to identify neurons. Kv1.1 exclusively labelled the cells identified as neurons, but not all of the NF200-stained neurons were co-labelled with Kv1.1. Ac, double exposure of the Kv1.1 and NF200 fluorescent images shown above. The arrow in each panel (a-c) shows an example of a cell identified with NF200 that did not co-label with Kv1.1. The calibration bar in the lower right of the figure equals 20 μm and applies to all of the panels. B, example traces from a voltage-clamp recording made from an acutely isolated spiral ganglion neuron under control conditions and after exposure to 100 nm DTX-K. The scale bar applies to both sweeps. C, APmax was significantly greater following exposure to DTX-K. Asterisks indicate a statistically significant difference between control and DTX-K treated neurons (n = 5, P < 0.01). D, an average activation curve was calculated from measured current and injected voltage levels from five acute experiments assuming a reversal potential of −80 mV. Data were normalized to the maximum conductance of the low voltage activated component. Boltzmann fits were made as described in the legend for Fig. 6. For the single fit, V1/2 (half-maximal voltage) was −59 mV and k (slope) was 5.7. The 95 % confidence intervals for these two parameters were −61 to −57 mV and 3.9 to 7.5, respectively. For the double fit, the V1/2 (half-maximal voltage) values were −59.5 and 0.1 mV, for the low and high voltage fits, respectively (95 % confidence intervals: −61.7 to −57.3 mV and −3.0 to 3.2). The k values were 5.3 and 7.6 for the low and high voltage fits, respectively (95 % confidence intervals: 3.5 to 7.1 and 4.6 to 10.6).

Article Snippet: α-DTX and DTX-K were obtained from Alomone Labs (Jerusalem, Israel) and used at a concentration of 100 n m to produce maximal block of Kv1.1 ( Owen et al. 1997 ).

Techniques: Marker, Staining, Isolation, Control, Activation Assay, Injection, Immunofluorescence, Patch Clamp